Critical Review Essay Example | Topics and Well Written Essays - 750 words - 4

Critical Review - Essay Example The main aim of this research is to investigate hCGÃŽ ² triggered signaling pathways and the linkage between hCGÃŽ ² expression and cell motility in the cancerous cells. The study aims to understand more on hCGÃŽ ². This aids the formulation of a study that may help in understanding molecular therapy in treating prostate cancer. Various methods are used in the treatment of cancer patients although they have not been successful in reducing the level of mortality rate among cancer patients (Chiu et al., 2012). Molecular therapy is a new method that takes a different approach to treat cancer since the carcinoma regenerates and as a result attacking the tumors is therefore only for the short-term making it impossible to stop the problem (Armstrong and George, 2008). The human chorionic gonadotropin ÃŽ ² (hCGÃŽ ²) induced migration and invasion in human prostate cancer cells is one of the methods although the molecular mechanisms are unclear. hCGÃŽ ² is therefore studied as the main trigger and its effects to the ERK 1/2 and MMPs studied to understand its effect on these regulators in order to come up with a conclusive argument as to whether the molecular therapy method is feasible in treating prostate cancer. The transfection method that involved establishing a stable cell line overexpressing hCGÃŽ ² in DU145 cells revealed that; the cells without integration were of hCGÃŽ ². The genes were dead and floating in the medium and the single colonies which stably express hCGÃŽ ² were collected (Babykutty et al., 2012). The method was used in dividing the cells and collecting cultured cells that could be used in other procedures as the process took place for two weeks. Typically, the dead cells from the experiment were not found (Leahy et al., 2012). The real time PCR separating the total RNA ensured that the culture cells remaining in the experiment